Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1) CLIA Kit
The Human CYFRA 21-1 (Cytokeratin Fragment Antigen 21-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of CYFRA 21-1 levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an invaluable tool for a wide range of research applications.CYFRA 21-1 is a fragment of cytokeratin-19, a protein found in epithelial cells, and is a well-established biomarker for various cancers, especially lung cancer.
Elevated levels of CYFRA 21-1 have been associated with poor prognosis and disease progression, making it a valuable marker for monitoring cancer patients and evaluating the effectiveness of treatment.Whether studying cancer biology, monitoring disease progression, or evaluating therapeutic interventions, the Human CYFRA 21-1 CLIA Kit provides researchers with a robust and efficient tool for advancing their understanding of cancer and developing improved diagnostic and treatment strategies.
Product Name:
Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1) CLIA Kit
SKU:
HUES01108
Target:
Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYFRA21-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYFRA21-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYFRA21-1. You can calculate the concentration of Human CYFRA21-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
84-98
88-102
Average (%)
96
90
95
1:4
Range (%)
87-98
83-96
90-105
Average (%)
92
90
96
1:8
Range (%)
98-112
104-116
85-98
Average (%)
106
110
91
1:16
Range (%)
99-116
93-105
89-103
Average (%)
106
99
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
99-115
105
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.24
487.32
1605.18
197.28
481.1
1455.28
Standard deviation
25.65
52.97
174.48
24.52
55.28
113.8
C V (%)
12.56
10.87
10.87
12.43
11.49
7.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYFRA21-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYFRA21-1 in samples. No significant cross-reactivity or interference between Human CYFRA21-1 and analogues was observed.
