Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1) ELISA Kit
The Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1) ELISA Kit is a valuable tool for the precise measurement of CYFRA21-1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.CYFRA21-1 is a fragment of cytokeratin found in epithelial cells, with elevated levels often associated with various cancers, particularly lung cancer. Monitoring CYFRA21-1 levels can aid in early detection, prognosis assessment, and treatment monitoring for cancer patients.
Additionally, it may also serve as a useful biomarker for other conditions such as inflammatory diseases and renal disorders.By utilizing the Human CYFRA21-1 ELISA Kit, researchers can gain valuable insights into the role of CYFRA21-1 in disease pathogenesis and potentially identify new therapeutic targets for improved patient outcomes. This kit is a reliable and efficient tool for studying the implications of CYFRA21-1 in various disease states and advancing biomedical research.
Product Name:
Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1) ELISA Kit
SKU:
HUES02988
Target:
Human CYFRA21-1 (Cytokeratin Fragment Antigen 21-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYFRA21-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYFRA21-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CYFRA21-1. You can calculate the concentration of Human CYFRA21-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
85-101
91-105
Average (%)
97
92
98
1:4
Range (%)
87-100
85-98
88-102
Average (%)
92
91
95
1:8
Range (%)
89-104
80-91
88-100
Average (%)
96
86
95
1:16
Range (%)
92-105
79-92
81-95
Average (%)
99
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-109
99
EDTA plasma (n=5)
96-109
101
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.49
9.48
1.11
2.33
10.1
Standard deviation
0.07
0.12
0.45
0.06
0.12
0.34
C V (%)
6.73
4.82
4.75
5.41
5.15
3.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CYFRA21-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYFRA21-1 in samples. No significant cross-reactivity or interference between Human CYFRA21-1 and analogues was observed.
