Human CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) CLIA Kit
The Human CYP24A1 (Cytochrome P450 Family 24 Subfamily A Polypeptide 1) CLIA Kit is a cutting-edge assay designed for the sensitive and accurate detection of CYP24A1 levels in human samples. This kit offers high specificity and precision, ensuring reliable and consistent results for a variety of research applications.CYP24A1 is an important enzyme involved in the metabolism of vitamin D, playing a crucial role in maintaining calcium homeostasis and bone health.
Abnormalities in CYP24A1 expression have been linked to various disorders such as hypercalcemia, osteoporosis, and certain types of cancer, underscoring the significance of this enzyme as a potential biomarker for disease diagnosis and treatment evaluation.With its advanced technology and reliable performance, the Human CYP24A1 CLIA Kit is an indispensable tool for researchers and healthcare professionals studying the role of CYP24A1 in health and disease.
Product Name:
Human CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) CLIA Kit
SKU:
HUES00271
Target:
Human CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP24A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP24A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP24A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYP24A1. You can calculate the concentration of Human CYP24A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
89-105
91-101
Average (%)
98
96
96
1:4
Range (%)
87-98
88-101
95-109
Average (%)
93
95
102
1:8
Range (%)
92-107
98-114
92-105
Average (%)
99
105
98
1:16
Range (%)
86-98
101-118
93-104
Average (%)
92
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.39
206.82
902.96
96.54
197.55
857.08
Standard deviation
11.45
15.02
84.61
12.38
16.38
64.45
C V (%)
10.86
7.26
9.37
12.82
8.29
7.52
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYP24A1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human CYP24A1 in samples. No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed.
