The Human Cyt-C (Cytochrome C) ELISA Kit is specifically designed for the precise measurement of cytochrome C levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Cytochrome C is a key component in the electron transport chain, playing a vital role in mitochondrial function and cell apoptosis. Dysregulation of cytochrome C has been linked to various diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a biomarker for studying disease mechanisms and potential therapeutic interventions.
With its reliable performance and ease of use, the Human Cyt-C (Cytochrome C) ELISA Kit is an invaluable tool for researchers looking to investigate the role of cytochrome C in health and disease.
Product Name:
Human Cyt-C (Cytochrome C) ELISA Kit
SKU:
HUES01343
Target:
Human Cyt-C (Cytochrome C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cyt-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cyt-C. You can calculate the concentration of Human Cyt-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
100-113
91-105
Average (%)
106
106
96
1:4
Range (%)
87-98
83-94
85-98
Average (%)
92
89
91
1:8
Range (%)
91-104
82-96
89-101
Average (%)
97
89
94
1:16
Range (%)
91-104
86-101
87-102
Average (%)
96
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
251.65
787.9
2006.56
250.23
755.47
1892.02
Standard deviation
12.99
40.97
82.87
16.64
34.52
79.84
C V (%)
5.16
5.2
4.13
6.65
4.57
4.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Cyt-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cyt-C in samples. No significant cross-reactivity or interference between Human Cyt-C and analogues was observed.
