The Human D2D (D-Dimer) ELISA Kit is meticulously tailored for the precise and quantitative measurement of D-Dimer levels in a variety of human biological samples.
D-Dimer serves as a critical biomarker in the assessment of the cross-linked fibrin degradation product, making it a vital tool for diagnosing and monitoring conditions associated with thrombosis, fibrinolysis, and coagulation activation. Our ELISA kit features exceptional sensitivity and specificity, ensuring accurate and reproducible outcomes essential for the comprehensive evaluation of hemostasis-related disorders. With a commitment to stringent quality control protocols, this assay kit delivers dependable performance and is designed for ease of use, making it an optimal solution for your research endeavors. Trust in our D2D ELISA Kit for robust and reliable quantification of this essential biomarker in your investigative studies.
Product Name:
Human D2D (D-Dimer) ELISA Kit
SKU:
AEES00156
Target:
Human D2D (D-Dimer)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human D2D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human D2D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human D2D. You can calculate the concentration of Human D2D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
-
85-97
-
Average (%)
-
92
-
1:4
Range (%)
-
84-99
-
Average (%)
-
90
-
1:8
Range (%)
-
87-102
-
Average (%)
-
93
-
1:16
Range (%)
-
85-98
-
Average (%)
-
92
-
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
-
-
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
-
-
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.12
42.85
134.39
19.2
39.8
143.76
Standard deviation
1.33
2.11
4.26
1.09
2.2
5.15
C V (%)
6.93
4.93
3.17
5.67
5.53
3.58
Sample type &Sample volume:
plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human D2D concentrations in plasma.
Specificity:
This kit recognizes Human D2D in samples. No significant cross-reactivity or interference between Human D2D and analogues was observed.
