The Human DBP (Vitamin D Binding Protein) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of DBP levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent choice for a variety of research applications.DBP, also known as Gc-globulin, is a key protein involved in the transport of vitamin D in the bloodstream and plays a crucial role in regulating the bioavailability of vitamin D metabolites.
Changes in DBP levels have been associated with various health conditions, including vitamin D deficiency, kidney disease, and autoimmune disorders.By accurately quantifying DBP levels, researchers can gain valuable insights into the role of this protein in health and disease, potentially leading to new diagnostic and therapeutic strategies. With its high performance and ease of use, the Human DBP CLIA Kit is a valuable tool for studying the complex relationships between DBP and human health.
Product Name:
Human DBP (Vitamin D Binding Protein) CLIA Kit
SKU:
HUES00914
Target:
Human DBP (Vitamin D Binding Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DBP. You can calculate the concentration of Human DBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
101-117
86-98
Average (%)
95
109
92
1:4
Range (%)
92-103
100-116
90-103
Average (%)
98
107
96
1:8
Range (%)
93-105
87-100
97-111
Average (%)
99
94
104
1:16
Range (%)
99-110
86-102
96-109
Average (%)
104
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.68
6.65
21.26
2.46
6.47
23.06
Standard deviation
0.35
0.56
1.71
0.3
0.52
2.38
C V (%)
13.06
8.42
8.04
12.2
8.04
10.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed.
