The Human DCN (Decorin) ELISA Kit is specifically designed for the precise measurement of decorin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it an indispensable tool for various research purposes.Decorin is a key proteoglycan that regulates various biological processes, such as collagen fibrillogenesis and cell growth.
Its involvement in tissue remodeling and inflammation makes it a valuable biomarker for studying conditions like fibrosis, arthritis, and cancer progression. By using this ELISA kit, researchers can gain valuable insights into the role of decorin in disease pathogenesis and explore potential therapeutic strategies.
Product Name:
Human DCN (Decorin) ELISA Kit
SKU:
HUES03134
Target:
Human DCN (Decorin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DCN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DCN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DCN. You can calculate the concentration of Human DCN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
96-111
98-113
Average (%)
98
104
106
1:4
Range (%)
91-102
83-95
83-94
Average (%)
97
90
89
1:8
Range (%)
90-102
82-97
86-99
Average (%)
96
89
93
1:16
Range (%)
92-103
79-92
84-96
Average (%)
98
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.71
11.96
26.86
3.65
12.24
26.31
Standard deviation
0.2
0.7
0.9
0.22
0.69
0.95
C V (%)
5.39
5.85
3.35
6.03
5.64
3.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DCN in samples. No significant cross-reactivity or interference between Human DCN and analogues was observed.
