The Human DCX (Doublecortin) CLIA Kit is a powerful tool for measuring levels of doublecortin in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for researchers studying neurogenesis and neuronal development.Doublecortin is a key protein involved in neuronal migration and differentiation, making it essential for proper brain development. Abnormalities in doublecortin expression have been linked to various neurological disorders, including epilepsy and autism spectrum disorders.
By accurately measuring doublecortin levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.This CLIA kit from Assay Genie offers a convenient and efficient solution for quantifying doublecortin levels in human samples, making it a valuable tool for neuroscience research and drug development efforts.
Product Name:
Human DCX (Doublecortin) CLIA Kit
SKU:
HUES01094
Target:
Human DCX (Doublecortin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DCX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DCX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DCX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DCX. You can calculate the concentration of Human DCX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
96-106
97-112
Average (%)
101
101
103
1:4
Range (%)
101-116
101-117
98-116
Average (%)
107
108
106
1:8
Range (%)
94-106
98-115
98-113
Average (%)
100
105
103
1:16
Range (%)
99-113
88-102
101-116
Average (%)
107
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
103
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.62
275.06
823.23
101.23
270.65
802.55
Standard deviation
11.98
25.17
49.72
12.64
28.12
75.76
C V (%)
12.27
9.15
6.04
12.49
10.39
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DCX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DCX in samples. No significant cross-reactivity or interference between Human DCX and analogues was observed.
