The Human DDC (Dopamine Decarboxylase) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of DDC levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.DDC is an important enzyme involved in the conversion of dopamine to norepinephrine, playing a key role in neurotransmitter production and regulation. Dysregulation of DDC activity has been implicated in various neurological disorders such as Parkinson's disease and schizophrenia.
Therefore, monitoring DDC levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human DDC CLIA Kit offers researchers a valuable tool for studying the role of DDC in health and disease, with the potential to contribute to advancements in diagnostic and treatment strategies.
Product Name:
Human DDC (Dopamine Decarboxylase) CLIA Kit
SKU:
HUES00700
Target:
Human DDC (Dopamine Decarboxylase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DDC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DDC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DDC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DDC. You can calculate the concentration of Human DDC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
99-111
84-98
Average (%)
99
105
90
1:4
Range (%)
89-104
99-117
88-101
Average (%)
96
107
94
1:8
Range (%)
83-95
89-105
89-102
Average (%)
90
96
94
1:16
Range (%)
101-117
97-111
85-101
Average (%)
109
103
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
101-116
108
Cell culture media (n=5)
99-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.82
236.58
706.22
97.54
220.62
756.44
Standard deviation
12.89
17.46
54.1
8.55
20.94
47.66
C V (%)
12.79
7.38
7.66
8.77
9.49
6.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DDC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DDC in samples. No significant cross-reactivity or interference between Human DDC and analogues was observed.
