The Human Defensin Beta 126 (DEFB126) ELISA Kit from AssayGenie is a cutting-edge tool for the accurate measurement of DEFB126 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with reliable and reproducible results, making it an invaluable asset for a variety of research applications.DEFB126 is a vital component of the human immune system, playing a key role in defense against microbial infections. Its dysregulation has been implicated in various inflammatory disorders, making it a promising target for therapeutic interventions.
By accurately quantifying DEFB126 levels, researchers can gain valuable insights into immune responses and potential treatment strategies for a range of diseases.In summary, the Human Defensin Beta 126 (DEFB126) ELISA Kit offers the precision and reliability needed for studying the role of DEFB126 in immunity and inflammatory pathways, paving the way for innovative research and therapeutic developments.
Product Name:
Human Defensin Beta 126 (DEFb126) ELISA Kit
Product Code:
HUDL00816
Size:
96 Assays
Target:
DEFb126
Synonyms:
ESP13.2, C20orf8, DEFB-26, Epididymal secretory protein 13.2
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
