The Human DKK1 (Dickkopf-related protein 1) CLIA Kit is a high-quality assay designed for the precise measurement of DKK1 levels in human samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.DKK1 is a key regulator of the Wnt signaling pathway and is involved in various biological processes, such as embryonic development, tissue homeostasis, and tumorigenesis. Abnormal DKK1 levels have been implicated in a range of diseases, including cancer, osteoporosis, and Alzheimer's disease, highlighting its importance as a potential diagnostic and therapeutic target.
With its easy-to-follow protocol and reliable performance, the Human DKK1 CLIA Kit is a valuable tool for scientists and researchers investigating the role of DKK1 in disease pathogenesis and evaluating its potential as a biomarker for disease detection and monitoring.
Product Name:
Human DKK1 (Dickkopf Related Protein 1) CLIA Kit
SKU:
HUES00055
Target:
Human DKK1 (Dickkopf Related Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DKK1. You can calculate the concentration of Human DKK1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
94-106
100-114
Average (%)
95
101
107
1:4
Range (%)
89-104
97-113
89-106
Average (%)
95
103
96
1:8
Range (%)
92-106
88-104
96-111
Average (%)
97
95
103
1:16
Range (%)
95-111
94-107
93-105
Average (%)
101
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
98-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.78
122.59
402.66
48.04
120.46
388.48
Standard deviation
5.46
14.36
32.66
4.44
12.94
31.62
C V (%)
11.93
11.71
8.11
9.24
10.74
8.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DKK1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DKK1 in samples. No significant cross-reactivity or interference between Human DKK1 and analogues was observed.
