The Human DKK4 (Dickkopf-related protein 4) CLIA Kit is specially designed for the accurate quantification of DKK4 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications.DKK4 is a key protein involved in the regulation of Wnt signaling pathway, which plays a critical role in cellular development, proliferation, and differentiation.
Dysregulation of this pathway has been linked to various diseases including cancer, osteoporosis, and Alzheimer's disease, making DKK4 a valuable biomarker for studying these conditions and potential therapeutic targets.With the Human DKK4 CLIA Kit from Assay Genie, researchers can accurately measure DKK4 levels to advance their understanding of disease mechanisms and explore new treatment options. Upgrade your research with this innovative and reliable assay kit today.
Product Name:
Human DKK4 (Dickkopf Related Protein 4) CLIA Kit
SKU:
HUES00513
Target:
Human DKK4 (Dickkopf Related Protein 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DKK4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DKK4. You can calculate the concentration of Human DKK4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
87-101
97-113
Average (%)
90
93
103
1:4
Range (%)
87-100
89-102
92-106
Average (%)
93
97
97
1:8
Range (%)
92-105
98-114
87-101
Average (%)
97
106
92
1:16
Range (%)
93-104
97-110
92-106
Average (%)
98
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
97-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.24
319.22
869.44
102.31
299.6
812.03
Standard deviation
10.74
37.09
81.55
12.56
32.99
57.98
C V (%)
10.61
11.62
9.38
12.28
11.01
7.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DKK4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DKK4 in samples. No significant cross-reactivity or interference between Human DKK4 and analogues was observed.
