The Human DS (Dermatan Sulfate) CLIA Kit is a highly sensitive and specific assay designed for the quantification of dermatan sulfate levels in human samples. This kit provides reliable and reproducible results, making it suitable for various research applications related to dermatan sulfate and its biological functions.Dermatan sulfate is a key component of the extracellular matrix and plays a vital role in maintaining tissue structure and function. Abnormal levels of dermatan sulfate have been associated with various diseases and conditions, including connective tissue disorders and cardiovascular diseases.
Therefore, accurate measurement of dermatan sulfate levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Human DS CLIA Kit is an essential tool for researchers studying dermatan sulfate biology and its implications in health and disease. Get your kit today and unlock new possibilities in your research endeavors.
Product Name:
Human DS (Dermatan Sulfate) CLIA Kit
SKU:
HUES00964
Target:
Human DS (Dermatan Sulfate)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DS. You can calculate the concentration of Human DS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-103
102-118
Average (%)
98
96
109
1:4
Range (%)
93-106
102-118
99-113
Average (%)
99
108
105
1:8
Range (%)
97-115
91-103
93-110
Average (%)
105
97
100
1:16
Range (%)
94-105
102-117
91-103
Average (%)
99
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.65
360.49
1523.35
205.39
343.98
1374.12
Standard deviation
20.11
35.26
173.81
21.4
34.29
120.92
C V (%)
10.33
9.78
11.41
10.42
9.97
8.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DS in samples. No significant cross-reactivity or interference between Human DS and analogues was observed.
