Description
Human Dynorphin A PharmaGenie ELISA Kit
| Product SKU: | SBRS0021 |
| Size: | 96T |
| Application: | This ELISA kit is designed to detect human, mouse, and rat Dynorphin. |
| Uniprot: | P01213 |
| Gene ID: | 5173 |
| Gene Names: | PDYN |
| Synonyms: | Proenkephalin-B (Beta-neoendorphin-dynorphin) (Preprodynorphin) [Cleaved into: Alpha-neoendorphin Beta-neoendorphin Big dynorphin (Big Dyn) Dynorphin A(1-17) (Dyn-A17) (Dynorphin A) Dynorphin A(1-13) Dynorphin A(1-8) Leu-enkephalin Rimorphin (Dynorphin B) (Dyn-B) (Dynorphin B(1-13)) Leumorphin (Dynorphin B-29)] |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 0.3 ng/ml |
| Recommended Dilution: | Human: 8X, Mouse: 8X, Rat: 8X |
| Storage/Stability: | Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- Technical Manual
Other materials and equipment required:
The Assay Genie Human Dynorphin A PharmaGenie ELISA Kit (SBRS0021) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Prepare all reagents, samples and standards as instructed.
- Add 100 ul detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 ul standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 ul prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 ul TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul Stop Solution to each well.
- Read plate at 450 nm immediately.
 | Standard Curve for Human Dynorphin A PharmaGenie ELISA Kit (SBES0021) |
Dynorphin A is a 17-amino acid opioid peptide that is cleaved from the precursor protein prodynorphin. It exerts its effects primarily through the k-opioid receptor (KOR), a G-protein-coupled receptor. Dynorphin has many different physiological actions. It has been shown to regulate pain response, cocaine addiction, Stress, depression, appetite control, circadian rhythms and body temperature.
