Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
The Human ECF (CCL11/Eosinophil Chemotactic Factor) ELISA Kit is a robust and reliable tool for the quantitative measurement of ECF levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ECF, also known as CCL11, is a chemokine that plays a crucial role in recruiting eosinophils to sites of inflammation and is involved in allergic responses. Elevated levels of ECF have been linked to conditions such as asthma, allergic rhinitis, and inflammatory diseases, making it a valuable biomarker for studying these conditions and evaluating treatment responses.
With its advanced technology and user-friendly design, the Human ECF ELISA Kit from Assay Genie provides researchers with a powerful tool for studying the role of ECF in various physiological and pathological processes. Order yours today and unlock the potential of ECF research.
Product Name:
Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
SKU:
HUES01312
Target:
Human ECF/CCL11 (Eosinophil Chemotactic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECF/CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ECF/CCL11. You can calculate the concentration of Human ECF/CCL11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
95-111
100-116
Average (%)
96
102
106
1:4
Range (%)
101-114
82-94
95-108
Average (%)
107
89
102
1:8
Range (%)
99-112
85-97
93-104
Average (%)
106
90
98
1:16
Range (%)
99-115
85-95
94-106
Average (%)
105
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.3
124.7
336.3
46.8
127.7
341.4
Standard deviation
2.6
5.1
10.1
2.9
6.4
16.4
C V (%)
5.07
4.09
3.0
6.2
5.01
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ECF/CCL11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed.
