The Human EG-VEGF (Endocrine Gland-Derived Vascular Endothelial Growth Factor) CLIA Kit is a cutting-edge tool designed for the precise measurement of EG-VEGF levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable asset for various research applications.EG-VEGF is a crucial growth factor that plays a pivotal role in angiogenesis, the process of new blood vessel formation. It is involved in regulating vascular development and function, making it a key player in various physiological processes.
Dysregulation of EG-VEGF has been implicated in diseases such as cancer, cardiovascular disorders, and other vascular-related conditions, highlighting its importance as a biomarker for disease research and therapeutic development.With its advanced technology and reliability, the Human EG-VEGF CLIA Kit offers researchers a powerful tool to explore the role of EG-VEGF in health and disease, paving the way for new insights and potential therapeutic targets.
Human EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EG-VEGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EG-VEGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EG-VEGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EG-VEGF. You can calculate the concentration of Human EG-VEGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
101-119
100-117
Average (%)
109
109
107
1:4
Range (%)
88-98
101-117
90-102
Average (%)
93
109
97
1:8
Range (%)
94-108
91-104
88-102
Average (%)
101
98
95
1:16
Range (%)
94-111
90-106
91-104
Average (%)
101
97
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
100-116
108
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.41
58.22
174.39
27.6
55.19
160.82
Standard deviation
2.13
5.75
16.2
2.92
6.53
14.52
C V (%)
8.38
9.88
9.29
10.58
11.83
9.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EG-VEGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EG-VEGF in samples. No significant cross-reactivity or interference between Human EG-VEGF and analogues was observed.
