Human EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
The Human EGFL7 (EGF-Like Domain Protein Multiple 7) ELISA Kit is specifically designed for the accurate detection of EGFL7 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.EGFL7 is an important protein that belongs to the epidermal growth factor (EGF) repeat superfamily. It plays a crucial role in angiogenesis, the process of forming new blood vessels from pre-existing ones. EGFL7 is involved in regulating endothelial cell proliferation, migration, and tube formation, making it a key player in vascular development and homeostasis.
Given its significance in angiogenesis and vascular biology, EGFL7 serves as a valuable biomarker for studying diseases related to abnormal blood vessel formation, such as cancer, cardiovascular diseases, and inflammation. By detecting and quantifying EGFL7 levels, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human EGFL7 ELISA Kit provides researchers with a reliable tool for measuring EGFL7 expression levels, furthering our understanding of angiogenesis and its implications in various diseases.
Product Name:
Human EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
Product Code:
HUDL00921
Size:
96 Assays
Target:
EGFL7
Synonyms:
ZNEU1, MEGF7, Zneu1, Vascular endothelial statin, EGF Like Domain Containing 7, Epidermal growth factor-like protein 7, Multiple epidermal growth factor-like
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
