Human EGFR2 (Epidermal Growth Factor Receptor 2) CLIA Kit
The Human EGFR2 (Epidermal Growth Factor Receptor 2) CLIA Kit is a cutting-edge assay designed for the precise measurement of EGFR2 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for a variety of research purposes.EGFR2, also known as HER2, is a vital protein that plays a crucial role in cell growth and proliferation. Dysregulation of EGFR2 has been linked to various diseases, including breast cancer and other forms of cancer.
Understanding the expression levels of EGFR2 can provide valuable insights into disease progression and potential treatment strategies.By utilizing the Human EGFR2 CLIA Kit, researchers can gain valuable information about EGFR2 levels in biological samples, enabling them to investigate its role in disease pathology and explore novel therapeutic interventions. This kit offers a comprehensive solution for studying EGFR2, making it an invaluable asset in the field of biomedical research.
Product Name:
Human EGFR2 (Epidermal Growth Factor Receptor 2) CLIA Kit
SKU:
HUES00925
Target:
Human EGFR2 (Epidermal Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EGFR2. You can calculate the concentration of Human EGFR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
93-107
101-116
Average (%)
91
100
108
1:4
Range (%)
102-115
87-101
95-110
Average (%)
108
94
103
1:8
Range (%)
98-112
97-112
94-106
Average (%)
105
103
99
1:16
Range (%)
101-116
93-104
85-97
Average (%)
107
99
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.43
175.15
879.27
101.7
184.38
831.91
Standard deviation
11.14
14.36
76.23
12.34
15.4
89.51
C V (%)
11.92
8.2
8.67
12.13
8.35
10.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EGFR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGFR2 in samples. No significant cross-reactivity or interference between Human EGFR2 and analogues was observed.
