Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit
The Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit is meticulously crafted to enable the precise and quantitative detection of EGFR2 levels in diverse human biological samples.
Epidermal Growth Factor Receptor 2 is a critical biomarker known for its role in cell proliferation, differentiation, and various cellular processes, particularly in cancer development and progression. Accurate quantification of EGFR2 is crucial for understanding its involvement in tumorigenesis, prognostic evaluation, and the development of targeted therapeutic strategies. Our ELISA kit offers exceptional sensitivity and specificity, ensuring robust and reproducible results in measuring EGFR2 concentrations. With stringent quality control measures in place, this kit guarantees reliable performance and ease of use, catering to both research investigations and clinical applications. Rely on Assay Genie's Human EGFR2 ELISA Kit for the accurate and dependable quantification of this pivotal biomarker in your scientific studies.
Product Name:
Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit
SKU:
AEES00119
Target:
Human EGFR2 (Epidermal Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EGFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human EGFR2. You can calculate the concentration of Human EGFR2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
85-97
94-104
Average (%)
93
92
99
1:4
Range (%)
83-98
98-110
97-109
Average (%)
91
105
105
1:8
Range (%)
89-100
96-109
82-98
Average (%)
92
102
90
1:16
Range (%)
93-107
93-102
88-100
Average (%)
99
98
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
98-107
102
Cell culture media (n=5)
96-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
80.78
209.27
875.31
82.01
202.03
907.19
Standard deviation
4.05
10.36
40.96
4.63
9.5
39.83
C V (%)
5.01
4.95
4.68
5.65
4.7
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human EGFR2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EGFR2 in samples. No significant cross-reactivity or interference between Human EGFR2 and analogues was observed.
