Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) CLIA Kit
The Human EMMPRIN (CD147) Extracellular Matrix Metalloproteinase Inducer CLIA Kit is specifically designed for the precise measurement of EMMPRIN levels in human biological samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research applications.EMMPRIN, also known as CD147, is a key protein involved in regulating the expression of matrix metalloproteinases, enzymes that play a crucial role in tissue remodeling and cancer progression. Studies have shown that EMMPRIN is overexpressed in various types of cancer and is associated with tumor invasion and metastasis.
Therefore, measuring EMMPRIN levels can provide valuable insights into cancer biology and potentially aid in the development of novel therapeutic strategies.With its cutting-edge technology and reliable performance, the Human EMMPRIN Extracellular Matrix Metalloproteinase Inducer CLIA Kit is an indispensable tool for researchers studying cancer biology, tumor progression, and potential therapeutic interventions. Order your kit today to advance your research and uncover new discoveries in the field of oncology.
Product Name:
Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) CLIA Kit
SKU:
HUES00916
Target:
Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EMMPRIN/CD147. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EMMPRIN/CD147 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EMMPRIN/CD147, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EMMPRIN/CD147. You can calculate the concentration of Human EMMPRIN/CD147 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
100-117
87-100
Average (%)
110
107
93
1:4
Range (%)
97-113
101-116
85-99
Average (%)
104
107
91
1:8
Range (%)
95-107
87-101
99-113
Average (%)
100
93
106
1:16
Range (%)
99-112
93-108
99-114
Average (%)
105
100
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
98-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.16
104.07
294.21
43.41
106.21
306.05
Standard deviation
4.73
8.94
26.98
5.53
7.54
21.85
C V (%)
11.49
8.59
9.17
12.74
7.1
7.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EMMPRIN/CD147 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EMMPRIN/CD147 in samples. No significant cross-reactivity or interference between Human EMMPRIN/CD147 and analogues was observed.
