Human ENA-78 (Epithelial Neutrophil Activating Peptide 78) CLIA Kit
The Human ENA-78 (Epithelial Neutrophil Activating Peptide 78) CLIA Kit is a cutting-edge assay designed for the precise quantification of ENA-78 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ENA-78 is a vital chemokine that plays a crucial role in inflammatory responses, leukocyte recruitment, and immune system regulation.
Elevated ENA-78 levels have been associated with various inflammatory conditions, including autoimmune diseases, asthma, and infections, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With its advanced technology and high-performance capabilities, the Human ENA-78 CLIA Kit is a valuable tool for researchers and scientists looking to delve deeper into the role of ENA-78 in human health and disease.
Product Name:
Human ENA-78 (Epithelial Neutrophil Activating Peptide 78) CLIA Kit
SKU:
HUES00045
Target:
Human ENA-78 (Epithelial Neutrophil Activating Peptide 78)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ENA-78/CXCL5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ENA-78/CXCL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ENA-78/CXCL5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ENA-78/CXCL5. You can calculate the concentration of Human ENA-78/CXCL5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
102-113
100-115
Average (%)
95
108
108
1:4
Range (%)
93-105
97-110
94-108
Average (%)
98
104
101
1:8
Range (%)
90-101
92-104
100-115
Average (%)
96
99
106
1:16
Range (%)
98-111
94-107
100-117
Average (%)
105
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-99
90
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.94
109.53
390.46
46.09
110.8
424.03
Standard deviation
3.78
7.86
26.75
4.02
11.72
47.02
C V (%)
8.05
7.18
6.85
8.72
10.58
11.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ENA-78/CXCL5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ENA-78/CXCL5 in samples. No significant cross-reactivity or interference between Human ENA-78/CXCL5 and analogues was observed.
