Human ER alpha (Estrogen Receptor Alpha) ELISA Kit
The Human ER alpha (Estrogen Receptor Alpha) ELISA Kit is specifically designed to detect levels of ER alpha in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.ER alpha is a key protein involved in mediating estrogen signaling, regulating gene expression, and influencing cellular growth and differentiation.
Abnormalities in ER alpha expression have been linked to various diseases such as breast cancer, osteoporosis, and cardiovascular disorders, making it a valuable biomarker for disease research and therapeutic development.With its reliable performance and broad utility, the Human ER alpha ELISA Kit is an essential tool for studying estrogen receptor biology and exploring its impact on health and disease. Get yours today at www.assaygenie.com!
Product Name:
Human ER alpha (Estrogen Receptor Alpha) ELISA Kit
SKU:
HUES02220
Target:
Human ER alpha (Estrogen Receptor Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ERα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ERα. You can calculate the concentration of Human ERα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
94-108
89-105
Average (%)
96
99
96
1:4
Range (%)
92-107
86-99
88-101
Average (%)
99
93
93
1:8
Range (%)
88-105
86-96
84-95
Average (%)
96
91
90
1:16
Range (%)
92-103
87-100
87-102
Average (%)
98
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.09
465.77
1900.81
203.53
448.94
1773.53
Standard deviation
12.02
26.08
89.15
12.6
26.26
53.38
C V (%)
6.26
5.6
4.69
6.19
5.85
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ERα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ERα in samples. No significant cross-reactivity or interference between Human ERα and analogues was observed.
