Human ER Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit
The Human ER-Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of ERLIN1 levels in human serum, plasma, and cell culture supernatants. ERLIN1 is a key protein involved in lipid raft function and endoplasmic reticulum-associated degradation (ERAD), playing a critical role in cellular processes related to protein quality control.This ELISA kit offers high specificity and reproducibility, ensuring precise and consistent results for a wide range of research applications.
ERLIN1 is implicated in various diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human ERLIN1 ELISA Kit is a powerful tool for researchers seeking to study the role of ERLIN1 in cellular processes and disease pathology, providing valuable insights for future research and treatment development.
Product Name:
Human ER Lipid Raft Associated Protein 1 (ERLIN1) ELISA Kit
Product Code:
HUDL00975
Size:
96 Assays
Target:
ERLIN1
Synonyms:
C10orf69, Erlin-1, KE04, KEO4, SPFH1, ER Lipid Raft Associated Protein 1, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
