Human Esophageal Cancer Related Gene 4 (ECRG4) ELISA Kit
The Human Esophageal Cancer-Related Gene 4 (ECRG4) ELISA Kit is a valuable tool for researchers studying esophageal cancer and related conditions. This kit is specifically designed for the accurate detection of ECRG4 levels in human serum, plasma, and cell culture supernatants.ECRG4 is a gene closely associated with esophageal cancer and has been linked to tumor suppression and anti-angiogenic properties. By measuring ECRG4 levels, researchers can better understand the mechanisms involved in esophageal cancer development and potentially identify new therapeutic targets.
With its high sensitivity and specificity, this ELISA kit ensures reliable and reproducible results, making it an essential tool for cancer research and drug development. Order your Human ECRG4 ELISA Kit today and take a step closer to unraveling the mysteries of esophageal cancer.
Product Name:
Human Esophageal Cancer Related Gene 4 (ECRG4) ELISA Kit
Product Code:
HUDL00908
Size:
96 Assays
Target:
ECRG4
Synonyms:
C2orf40, Augurin
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
