Human Estrogen Related Receptor Alpha (ERRa) ELISA Kit
The Human Estrogen Related Receptor Alpha (ERRα) ELISA Kit offered by Assay Genie is a powerful tool for the precise measurement of ERRα levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.ERRα is a key transcription factor that plays a critical role in the regulation of energy metabolism, mitochondrial biogenesis, and cell proliferation.
Dysregulation of ERRα has been linked to various diseases including cancer, metabolic disorders, and neurodegenerative conditions, making it a valuable biomarker for studying and potentially targeting these pathologies.In summary, the ERRα ELISA Kit from Assay Genie is an indispensable tool for researchers seeking to investigate the role of ERRα in health and disease, providing reliable data to advance our understanding and potentially develop new therapeutic interventions.
Product Name:
Human Estrogen Related Receptor Alpha (ERRa) ELISA Kit
Product Code:
HUDL00978
Size:
96 Assays
Target:
ERRa
Synonyms:
ERR1, ERRa, ERRalpha, ESRL1, NR3B1, Estrogen receptor-like 1, Nuclear receptor subfamily 3 group B member 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
