The Human Endothelin Receptor B CLIA Kit is a cutting-edge assay designed for the precise measurement of endothelin receptor B levels in human samples, including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Endothelin receptor B is a key player in the regulation of vascular tone and blood pressure, making it a vital target for understanding cardiovascular diseases and potential therapeutic interventions.
By accurately quantifying endothelin receptor B levels, researchers can gain valuable insights into the role of this receptor in various pathological conditions and develop targeted therapies for related disorders.Overall, the Human Endothelin Receptor B CLIA Kit is a valuable tool for researchers seeking to explore the role of endothelin receptor B in health and disease, providing a reliable and efficient method for studying this important protein.
Product Name:
Human ETRB (Endothelin Receptor B) CLIA Kit
SKU:
HUES00992
Target:
Human ETRB (Endothelin Receptor B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ETRB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ETRB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ETRB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ETRB. You can calculate the concentration of Human ETRB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
84-96
88-99
Average (%)
106
91
93
1:4
Range (%)
102-117
94-106
95-109
Average (%)
109
101
101
1:8
Range (%)
86-96
93-107
101-113
Average (%)
91
100
107
1:16
Range (%)
87-103
100-118
100-115
Average (%)
94
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.48
469.11
1836.27
198.84
457.77
1768.73
Standard deviation
18.85
40.91
198.5
22.01
46.51
159.01
C V (%)
9.95
8.72
10.81
11.07
10.16
8.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ETRB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ETRB in samples. No significant cross-reactivity or interference between Human ETRB and analogues was observed.
