Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
The Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of EAAT5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional specificity, ensuring precise and reproducible results for a variety of research applications.EAAT5 is a critical transporter protein involved in the regulation of excitatory amino acids in the central nervous system.
Dysregulation of EAAT5 has been linked to neurological disorders such as epilepsy and schizophrenia, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.With its superior performance and ease of use, the Human EAAT5 ELISA Kit is an essential tool for researchers seeking to explore the role of EAAT5 in neurological disorders and advance our understanding of excitatory amino acid transport mechanisms.
Product Name:
Human Excitatory Amino Acid Transporter 5 (EAAT5) ELISA Kit
Product Code:
HUDL00903
Size:
96 Assays
Target:
EAAT5
Synonyms:
SLC1A7, AAAT, Solute Carrier Family 1 Member 7,Glutamate Transporter, Retinal glutamate transporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
