The Human F1+2 Prothrombin Fragment 1-2 CLIA Kit is a cutting-edge assay designed for the precise and efficient measurement of levels of the F1+2 Prothrombin Fragment 1-2 in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.The F1+2 Prothrombin Fragment 1-2 is a key biomarker associated with blood coagulation and thrombosis. Its measurement can provide insights into clotting disorders, cardiovascular diseases, and other related conditions, making it a valuable tool for studying the mechanisms underlying these health issues and developing potential therapeutic interventions.
With its advanced technology and proven performance, the Human F1+2 Prothrombin Fragment 1-2 CLIA Kit is an essential addition to any laboratory focused on understanding coagulation processes and associated diseases. Trust in this kit to deliver precise and consistent results, advancing your research efforts in this important field.
Product Name:
Human F1+2 (Prothrombin Fragment 1+2) CLIA Kit
SKU:
HUES00995
Target:
Human F1+2 (Prothrombin Fragment 1+2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F1+2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F1+2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F1+2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F1+2. You can calculate the concentration of Human F1+2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
93-108
103-116
Average (%)
109
99
109
1:4
Range (%)
86-100
99-114
93-109
Average (%)
92
107
100
1:8
Range (%)
100-115
97-113
92-108
Average (%)
107
104
98
1:16
Range (%)
100-113
92-106
85-98
Average (%)
107
98
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.11
158.27
427.08
48.96
163.55
461.06
Standard deviation
5.16
13.04
35.36
3.99
15.32
27.76
C V (%)
11.19
8.24
8.28
8.15
9.37
6.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F1+2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F1+2 in samples. No significant cross-reactivity or interference between Human F1+2 and analogues was observed.
