The Human F7 (Coagulation Factor VII) CLIA Kit is specifically designed for the precise quantification of Coagulation Factor VII levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Coagulation Factor VII, also known as serum prothrombin conversion accelerator, is a key protein in the blood clotting cascade.
Dysregulation of this factor can lead to bleeding disorders or thrombotic events, making it a valuable biomarker for studying coagulation-related diseases and developing targeted therapies.With its ease of use and reliable performance, the Human F7 CLIA Kit from Assay Genie is an essential tool for researchers investigating coagulation disorders, cardiovascular diseases, and other conditions where Coagulation Factor VII plays a critical role.
Product Name:
Human F7 (Coagulation Factor VII) CLIA Kit
SKU:
HUES00475
Target:
Human F7 (Coagulation Factor VII)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F7. You can calculate the concentration of Human F7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
88-103
85-100
Average (%)
109
94
91
1:4
Range (%)
102-116
101-114
88-101
Average (%)
109
107
95
1:8
Range (%)
91-105
86-97
103-117
Average (%)
98
92
109
1:16
Range (%)
85-95
101-118
101-116
Average (%)
90
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.63
105.07
393.82
55.69
101.27
363.99
Standard deviation
4.37
8.8
27.92
6.2
10.84
24.53
C V (%)
8.15
8.38
7.09
11.13
10.7
6.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F7 in samples. No significant cross-reactivity or interference between Human F7 and analogues was observed.
