The Human F7 (Coagulation Factor VII) ELISA Kit is a reliable and accurate tool for measuring F7 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Coagulation Factor VII is a key protein in the blood coagulation cascade, playing a crucial role in the initiation of the coagulation process. Dysregulation of F7 levels can lead to bleeding disorders or thrombotic events, making it an important biomarker for studying coagulation disorders, cardiovascular diseases, and liver conditions.
By utilizing the Human F7 ELISA Kit, researchers can gain valuable insights into the role of Coagulation Factor VII in various disease states, paving the way for the development of novel therapeutic interventions and personalized medicine approaches.
Product Name:
Human F7 (Coagulation Factor VII) ELISA Kit
SKU:
HUES01901
Target:
Human F7 (Coagulation Factor VII)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human F7. You can calculate the concentration of Human F7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
88-98
86-99
Average (%)
99
93
91
1:4
Range (%)
101-116
84-94
96-109
Average (%)
107
89
101
1:8
Range (%)
96-114
82-94
95-110
Average (%)
104
87
102
1:16
Range (%)
93-108
81-93
96-108
Average (%)
100
86
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.7
356.3
1624.5
209.5
327.1
1650.2
Standard deviation
12.5
18.5
66.6
13.0
14.4
72.6
C V (%)
5.69
5.19
4.1
6.21
4.4
4.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human F7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F7 in samples. No significant cross-reactivity or interference between Human F7 and analogues was observed.
