Human FABP1 (Fatty Acid Binding Protein 1, Liver) CLIA Kit
The Human FABP1 (Fatty Acid Binding Protein 1) Liver CLIA Kit is a reliable and sensitive assay designed for the accurate quantification of FABP1 levels in human liver tissues. This kit offers high specificity and precision, ensuring robust and consistent results for a variety of research applications.FABP1 is an important protein involved in fatty acid metabolism and transport in the liver, playing a critical role in lipid homeostasis and energy balance.
Dysregulation of FABP1 levels has been associated with various liver diseases, including non-alcoholic fatty liver disease (NAFLD) and liver cancer. Therefore, this kit serves as a valuable tool for studying the pathophysiology of these conditions and exploring potential therapeutic interventions.
Product Name:
Human FABP1 (Fatty Acid Binding Protein 1, Liver) CLIA Kit
SKU:
HUES00067
Target:
Human FABP1 (Fatty Acid Binding Protein 1, Liver)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP1. You can calculate the concentration of Human FABP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
92-107
100-112
Average (%)
91
100
106
1:4
Range (%)
88-99
97-110
93-106
Average (%)
94
103
100
1:8
Range (%)
85-99
93-106
93-108
Average (%)
92
100
99
1:16
Range (%)
86-99
92-107
94-105
Average (%)
93
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.2
14.55
47.39
4.71
15.7
43.79
Standard deviation
0.57
1.14
4.74
0.5
1.86
4.67
C V (%)
10.96
7.84
10.0
10.62
11.85
10.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP1 in samples. No significant cross-reactivity or interference between Human FABP1 and analogues was observed.
