Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) CLIA Kit
The Human FABP4 (Fatty Acid Binding Protein 4) CLIA Kit is a powerful tool for accurately measuring FABP4 levels in human adipocyte samples. This kit offers high sensitivity and specificity, providing dependable and consistent results for various research purposes.FABP4 is a key protein involved in lipid metabolism and inflammation, playing a crucial role in conditions such as obesity, diabetes, and cardiovascular diseases.
By detecting and quantifying FABP4 levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Human FABP4 CLIA Kit is an indispensable resource for studying adipocyte biology and its implications in metabolic disorders. Order yours today and unlock the potential of FABP4 research.
Product Name:
Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) CLIA Kit
SKU:
HUES00212
Target:
Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP4. You can calculate the concentration of Human FABP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
102-119
83-97
Average (%)
98
108
90
1:4
Range (%)
92-109
89-101
95-111
Average (%)
100
94
102
1:8
Range (%)
93-107
97-112
88-102
Average (%)
99
102
94
1:16
Range (%)
96-110
93-109
90-105
Average (%)
103
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
101-118
108
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
231.78
499.34
1814.16
228.49
518.13
1728.9
Standard deviation
23.66
48.74
170.89
26.73
50.1
158.71
C V (%)
10.21
9.76
9.42
11.7
9.67
9.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed.
