Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3) CLIA Kit
The Human FAIM3 (Fas apoptotic inhibitory molecule 3) CLIA Kit is a powerful tool designed for the precise measurement of FAIM3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FAIM3 is a key protein that plays a critical role in inhibiting apoptosis, or programmed cell death, thereby promoting cell survival.
Dysregulation of FAIM3 has been implicated in various diseases, including cancer and autoimmune disorders, highlighting its importance as a potential therapeutic target and diagnostic biomarker.With its reliable performance and easy-to-use format, the Human FAIM3 CLIA Kit from Assay Genie is an essential tool for researchers studying apoptosis regulation and developing novel treatments for a range of diseases.
Product Name:
Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3) CLIA Kit
SKU:
HUES00268
Target:
Human FAIM3 (Fas Apoptotic Inhibitory Molecule 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FAIM3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FAIM3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FAIM3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FAIM3. You can calculate the concentration of Human FAIM3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
101-115
98-111
Average (%)
96
106
104
1:4
Range (%)
89-101
88-103
94-108
Average (%)
95
96
101
1:8
Range (%)
92-105
84-96
101-117
Average (%)
99
90
109
1:16
Range (%)
95-110
99-114
101-118
Average (%)
100
105
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.71
247.79
890.08
98.02
225.41
887.68
Standard deviation
9.65
18.68
73.97
9.83
22.95
55.12
C V (%)
10.08
7.54
8.31
10.03
10.18
6.21
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FAIM3 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human FAIM3 in samples. No significant cross-reactivity or interference between Human FAIM3 and analogues was observed.
