The Human FBLN3 (Fibulin-3) ELISA Kit is specifically designed for the precise measurement of fibulin-3 levels in human serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Fibulin-3, also known as FBLN3, is a key protein involved in extracellular matrix organization and cell adhesion. It plays a critical role in various physiological processes, including tissue development, wound healing, and cell migration.
Dysregulation of fibulin-3 has been implicated in conditions such as cancer, fibrosis, and cardiovascular diseases, underscoring its significance as a potential biomarker for disease diagnosis and therapeutic development.With its reliable performance and broad range of applications, the Human FBLN3 ELISA Kit is invaluable for researchers studying the role of fibulin-3 in health and disease. Trust in this kit to deliver accurate and meaningful data for your scientific investigations.
Product Name:
Human FBLN3 (Fibulin 3) ELISA Kit
SKU:
HUES02671
Target:
Human FBLN3 (Fibulin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FBLN3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBLN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FBLN3. You can calculate the concentration of Human FBLN3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
93-107
92-106
Average (%)
100
101
98
1:4
Range (%)
94-107
85-94
86-99
Average (%)
100
89
93
1:8
Range (%)
94-106
83-95
84-98
Average (%)
100
89
91
1:16
Range (%)
91-106
83-96
88-101
Average (%)
97
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
95-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.62
7.12
1.1
2.61
7.22
Standard deviation
0.07
0.13
0.23
0.06
0.12
0.34
C V (%)
6.73
4.96
3.23
5.45
4.6
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FBLN3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBLN3 in samples. No significant cross-reactivity or interference between Human FBLN3 and analogues was observed.
