The Human FBN1 (Fibrillin-1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of Fibrillin-1 levels in human samples, including serum, plasma, and cell culture supernatants. Fibrillin-1 is a key component of extracellular matrix microfibrils and plays a critical role in maintaining tissue structure and integrity.This kit offers accurate and reliable results, making it an excellent tool for researchers studying conditions such as Marfan syndrome, a genetic disorder characterized by mutations in the FBN1 gene.
By measuring Fibrillin-1 levels, this kit can provide valuable insights into the pathogenesis of Marfan syndrome and other related disorders, paving the way for the development of potential diagnostic and therapeutic strategies.Overall, the Human FBN1 (Fibrillin-1) CLIA Kit is a valuable resource for research aimed at understanding the role of Fibrillin-1 in health and disease, offering researchers a powerful tool to advance their studies in this important field.
Product Name:
Human FBN1 (Fibrillin 1) CLIA Kit
SKU:
HUES01183
Target:
Human FBN1 (Fibrillin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FBN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FBN1. You can calculate the concentration of Human FBN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
90-103
94-111
Average (%)
107
98
102
1:4
Range (%)
100-118
93-104
91-105
Average (%)
108
98
97
1:8
Range (%)
99-114
87-98
87-99
Average (%)
105
93
93
1:16
Range (%)
99-116
102-118
86-100
Average (%)
106
109
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.11
481.99
1963.81
204.03
517.48
2021.96
Standard deviation
23.38
38.08
134.52
19.65
42.28
215.54
C V (%)
11.4
7.9
6.85
9.63
8.17
10.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FBN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBN1 in samples. No significant cross-reactivity or interference between Human FBN1 and analogues was observed.
