The Human FG (Fibrinogen) CLIA Kit is specifically designed for the quantitative measurement of fibrinogen levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, providing researchers with reliable and consistent results for various research applications.Fibrinogen is a key protein involved in blood clotting and inflammation, playing a crucial role in cardiovascular diseases, thrombosis, and other related disorders. By accurately measuring fibrinogen levels, researchers can gain valuable insights into disease progression, treatment effectiveness, and potential therapeutic targets.
Overall, the Human FG (Fibrinogen) CLIA Kit is an essential tool for studying fibrinogen-related diseases and developing innovative strategies for diagnosis and management. Its advanced technology and usability make it a valuable asset for researchers seeking to deepen their understanding of fibrinogen biology and its implications in human health.
Product Name:
Human FG (Fibrinogen) CLIA Kit
SKU:
HUES01155
Target:
Human FG (Fibrinogen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FG. You can calculate the concentration of Human FG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
101-115
90-105
Average (%)
92
107
97
1:4
Range (%)
92-108
88-99
87-97
Average (%)
99
93
92
1:8
Range (%)
98-117
101-116
101-115
Average (%)
107
106
109
1:16
Range (%)
90-102
89-103
103-119
Average (%)
95
95
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11.08
21.96
94.6
10.2
21.66
89.54
Standard deviation
1.03
2.42
8.45
0.93
1.87
5.49
C V (%)
9.3
11.02
8.93
9.12
8.63
6.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FG in samples. No significant cross-reactivity or interference between Human FG and analogues was observed.
