The Human Fibrinogen (FG) ELISA Kit is specifically designed for the quantitative determination of fibrinogen levels in diverse human biological samples. Fibrinogen is a key protein involved in blood coagulation, playing a crucial role in hemostasis, clot formation, and wound healing processes. Accurate measurement of fibrinogen levels is essential for assessing coagulation disorders, monitoring cardiovascular diseases, and evaluating inflammatory responses. Our FG ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs.
Manufactured under stringent quality control guidelines, this kit guarantees robust performance and ease of use, making it a reliable choice for laboratory investigations. Trust Assay Genie's FG ELISA Kit for accurate and consistent quantification of this vital biomarker in your studies.
Product Name:
Human FG (Fibrinogen) ELISA Kit
SKU:
AEES00140
Target:
Human FG (Fibrinogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FG. You can calculate the concentration of Human FG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
85-95
91-106
Average (%)
101
92
99
1:4
Range (%)
101-110
98-104
84-98
Average (%)
105
101
90
1:8
Range (%)
98-112
83-97
87-99
Average (%)
104
91
95
1:16
Range (%)
99-112
93-103
95-106
Average (%)
105
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-97
92
EDTA plasma (n=5)
84-98
92
Cell culture media (n=5)
86-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.84
104.71
486.46
47.03
117.3
409.71
Standard deviation
2.56
4.86
23.98
2.62
5.3
19.01
C V (%)
5.71
4.64
4.93
5.57
4.52
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FG in samples. No significant cross-reactivity or interference between Human FG and analogues was observed.
