Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA Kit
The Human FK506 Binding Protein 12-Rapamycin Associated Protein (FRAP) ELISA Kit is a powerful tool for the precise measurement of FRAP levels in human biological samples. This kit is specifically designed to provide high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.FRAP, also known as mTOR (mechanistic target of rapamycin), is a key regulator of cell growth, metabolism, and autophagy. Dysregulation of mTOR signaling has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions.
Monitoring FRAP levels can offer valuable insights into the molecular mechanisms underlying these diseases and aid in the development of targeted therapies.By using the Human FK506 Binding Protein 12-Rapamycin Associated Protein (FRAP) ELISA Kit, researchers can efficiently analyze FRAP levels in serum, plasma, and cell culture supernatants, paving the way for advanced studies in the field of molecular biology and drug development.
Product Name:
Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA Kit
Product Code:
HUDL01108
Size:
96 Assays
Target:
FRAP
Synonyms:
FRAP1, MTOR, FRAP2, MTOR, RAFT1, RAPT1, Mammalian Target Of Rapamycin, FKBP12-Rapamycin Complex-Associated Protein 1, Rapamycin and FKBP12 target 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
