The Human FLNC (Filamin C gamma) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of FLNC in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results for a variety of research applications.FLNC is a key protein involved in cell structure and function, playing a role in muscle development and maintenance.
Dysregulation of FLNC has been linked to various diseases, including muscular dystrophy and heart conditions, making it a valuable biomarker for studying these disorders and developing new treatments.Overall, the Human FLNC CLIA Kit is a valuable resource for researchers interested in studying the role of FLNC in health and disease, providing accurate measurements and insightful data for further investigation.
Product Name:
Human FLNC (Filamin C, Gamma) CLIA Kit
SKU:
HUES01118
Target:
Human FLNC (Filamin C, Gamma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FLNC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FLNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FLNC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FLNC. You can calculate the concentration of Human FLNC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
92-103
94-106
Average (%)
102
97
99
1:4
Range (%)
86-99
90-104
90-105
Average (%)
91
95
97
1:8
Range (%)
90-104
95-109
96-111
Average (%)
96
101
103
1:16
Range (%)
100-116
88-100
101-116
Average (%)
107
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
97-113
105
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.2
470.83
1377.23
204.46
485.28
1312.2
Standard deviation
20.57
42.09
92.55
17.81
46.59
138.17
C V (%)
10.54
8.94
6.72
8.71
9.6
10.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FLNC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FLNC in samples. No significant cross-reactivity or interference between Human FLNC and analogues was observed.
