The Human Gal1 (Galectin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of Gal1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gal1 is a key protein involved in various biological processes, including cell adhesion, inflammation, and immune response modulation. Dysregulation of Gal1 has been implicated in diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
With its high sensitivity and specificity, the Human Gal1 CLIA Kit offers researchers a powerful tool for investigating the role of Gal1 in health and disease, ultimately advancing our understanding of its biological functions and potential clinical implications.
Product Name:
Human GAL1 (Galectin 1) CLIA Kit
SKU:
HUES00616
Target:
Human GAL1 (Galectin 1 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL1. You can calculate the concentration of Human GAL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
101-116
100-113
Average (%)
104
108
105
1:4
Range (%)
93-108
86-99
100-115
Average (%)
100
91
108
1:8
Range (%)
84-96
94-109
95-109
Average (%)
91
99
102
1:16
Range (%)
102-119
88-102
93-106
Average (%)
110
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.44
3.42
0.48
1.43
3.59
Standard deviation
0.06
0.15
0.27
0.06
0.11
0.22
C V (%)
11.54
10.42
7.89
12.5
7.69
6.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
