The Human Gal3 (Galectin-3) ELISA Kit is specifically designed for the precise measurement of Gal3 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Gal3, also known as galectin-3, is a key player in various biological processes, including inflammation, fibrosis, and cancer development.
Its overexpression has been linked to a number of diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Gal3 ELISA Kit offers a reliable and efficient method for measuring Gal3 levels, making it an indispensable tool for researchers in the field of biomarker discovery and disease diagnostics.
Product Name:
Human GAL3 (Galectin 3) ELISA Kit
SKU:
HUES02501
Target:
Human GAL3 (Galectin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL3. You can calculate the concentration of Human GAL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
93-110
86-96
Average (%)
103
101
91
1:4
Range (%)
94-108
81-92
88-99
Average (%)
100
86
93
1:8
Range (%)
89-104
86-96
80-94
Average (%)
95
91
87
1:16
Range (%)
88-102
84-97
83-99
Average (%)
94
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.03
3.99
0.54
0.96
3.64
Standard deviation
0.04
0.05
0.18
0.03
0.05
0.2
C V (%)
7.27
4.85
4.51
5.56
5.21
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
