The Human GC (Glucagon) ELISA Kit is specifically designed for the precise measurement of glucagon levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an excellent choice for various research applications.Glucagon is a hormone that plays a critical role in regulating blood sugar levels by promoting the release of glucose from the liver.
Imbalances in glucagon levels can lead to conditions such as diabetes and hypoglycemia, making it a valuable biomarker for studying metabolic disorders and developing new treatment strategies.Overall, the Human GC (Glucagon) ELISA Kit is a powerful tool for researchers looking to study the role of glucagon in health and disease, offering reliable and reproducible results for a wide range of experimental settings.
Product Name:
Human GC (Glucagon) ELISA Kit
SKU:
HUES03123
Target:
Human GC (Glucagon)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GC. During the reaction, GC in the sample or standard competes with a fixed amount of GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
87-100
94-107
Average (%)
105
94
99
1:4
Range (%)
90-104
87-101
97-113
Average (%)
96
94
103
1:8
Range (%)
89-103
92-102
95-111
Average (%)
95
97
101
1:16
Range (%)
89-103
89-105
97-112
Average (%)
95
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.1
209.1
817.7
86.8
218.3
757.1
Standard deviation
6.3
11.5
33.5
5.2
10.7
39.4
C V (%)
6.56
5.5
4.1
5.99
4.9
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GC in samples. No significant cross-reactivity or interference between GC and analogues was observed.
