Human GDF15 (Growth Differentiation Factor 15) CLIA Kit
The Human GDF15 (Growth Differentiation Factor 15) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GDF15 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.GDF15 is a protein that plays a crucial role in regulating various cellular processes, including cell growth, differentiation, and inflammation.
It is implicated in a range of diseases, such as cancer, cardiovascular diseases, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.With its advanced technology and easy-to-use format, the Human GDF15 CLIA Kit is an essential tool for researchers looking to investigate the role of GDF15 in health and disease. Order yours today from AssayGenie to elevate your research to the next level.
Product Name:
Human GDF15 (Growth Differentiation Factor 15) CLIA Kit
SKU:
HUES00078
Target:
Human GDF15 (Growth Differentiation Factor 15)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GDF15. You can calculate the concentration of Human GDF15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
89-103
86-100
Average (%)
100
95
94
1:4
Range (%)
96-109
92-105
96-113
Average (%)
102
98
103
1:8
Range (%)
96-110
90-107
96-107
Average (%)
104
98
101
1:16
Range (%)
98-114
87-100
86-99
Average (%)
106
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
107
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.25
74.9
217.76
21.93
74.73
209.4
Standard deviation
2.26
8.8
24.82
2.43
7.88
21.71
C V (%)
9.32
11.75
11.4
11.08
10.54
10.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GDF15 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
