Human GDF15 (Growth Differentiation Factor 15) ELISA Kit
The Human GDF15 (Growth Differentiation Factor 15) ELISA Kit is specially designed to accurately measure GDF15 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GDF15 is a key protein involved in regulating cell growth, differentiation, and inflammation. It has been implicated in various diseases including cancer, cardiovascular diseases, and metabolic disorders, making it a valuable biomarker for studying disease progression and identifying potential therapeutic targets. With its reliable performance and broad applicability, the Human GDF15 ELISA Kit from Assay Genie is an essential tool for researchers seeking to deepen their understanding of GDF15 biology and its role in human health and disease.
Product Name:
Human GDF15 (Growth Differentiation Factor 15) ELISA Kit
SKU:
HUES01367
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
