Human GDF2 (Growth Differentiation Factor 2) ELISA Kit
The Human GDF2 (Growth Differentiation Factor 2) ELISA Kit is meticulously developed for the precise measurement of Growth Differentiation Factor 2 (GDF2) levels in diverse biological samples.
GDF2, a member of the TGF-beta superfamily, acts as a vital signaling molecule in various cellular processes, including growth, differentiation, and development. Its involvement in key biological functions makes it a significant factor in tissue repair, cell regeneration, and pathophysiological conditions. Accurate quantification of GDF2 is essential for unraveling its roles in health and disease, providing valuable insights into potential therapeutic interventions. Our GDF2 ELISA Kit boasts outstanding sensitivity and specificity, guaranteeing accurate and reproducible outcomes. Crafted under strict quality control measures, this kit ensures robust performance and simple usability, making it an ideal solution for cutting-edge research endeavors. Rely on Assay Genie's GDF2 ELISA Kit for dependable quantification of this critical growth factor in your scientific investigations.
Product Name:
Human GDF2 (Growth Differentiation Factor 2) ELISA Kit
SKU:
AEES00165
Target:
Human GDF2 (Growth Differentiation Factor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF2. You can calculate the concentration of Human GDF2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
85-98
88-99
Average (%)
94
90
94
1:4
Range (%)
86-99
85-99
87-100
Average (%)
91
90
94
1:8
Range (%)
88-99
88-101
86-99
Average (%)
94
93
92
1:16
Range (%)
88-101
84-97
87-98
Average (%)
93
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.08
227.51
770.01
88.66
215.59
811.08
Standard deviation
6.55
12.35
25.8
5.01
9.01
37.15
C V (%)
6.96
5.43
3.35
5.65
4.18
4.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF2 in samples. No significant cross-reactivity or interference between Human GDF2 and analogues was observed.
