Human GHITM (Growth Hormone Inducible Transmembrane Protein) CLIA Kit
The Human Ghitm (Growth Hormone-Inducible Transmembrane Protein) CLIA Kit is a powerful tool for the quantitative measurement of Ghitm levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Ghitm is an important protein involved in growth hormone signaling and cellular growth processes.
It plays a key role in regulating growth and development, making it a valuable biomarker for studying growth-related disorders and potential therapeutic interventions.With this advanced CLIA kit, researchers can gain valuable insights into the role of Ghitm in various biological processes and diseases, paving the way for new discoveries and treatment options. Upgrade your research with the Human Ghitm CLIA Kit from AssayGenie today.
Product Name:
Human GHITM (Growth Hormone Inducible Transmembrane Protein) CLIA Kit
SKU:
HUES01060
Target:
Human GHITM (Growth Hormone Inducible Transmembrane Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GHITM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GHITM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHITM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GHITM. You can calculate the concentration of Human GHITM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
94-110
86-97
Average (%)
93
101
92
1:4
Range (%)
100-117
100-116
95-110
Average (%)
107
108
103
1:8
Range (%)
86-97
102-117
101-116
Average (%)
92
109
106
1:16
Range (%)
90-102
100-114
102-114
Average (%)
97
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
100-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.09
360.48
1529.81
201.38
354.1
1499.46
Standard deviation
16.15
39.8
122.69
19.39
26.38
155.94
C V (%)
8.32
11.04
8.02
9.63
7.45
10.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GHITM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GHITM in samples. No significant cross-reactivity or interference between Human GHITM and analogues was observed.
