The Human GL Alpha Galactosidase Alpha ELISA Kit is designed for the precise measurement of GL Alpha Galactosidase Alpha levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.GL Alpha Galactosidase Alpha is an important enzyme involved in the breakdown of complex carbohydrates, specifically alpha-galactosidase.
Deficiencies in this enzyme can lead to conditions such as Fabry disease, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.With easy-to-follow instructions and quick assay times, the Human GL Alpha Galactosidase Alpha ELISA Kit is an essential tool for researchers and clinicians looking to accurately measure GL Alpha Galactosidase Alpha levels in biological samples.
Product Name:
Human GL alpha (Galactosidase Alpha) ELISA Kit
SKU:
HUES02093
Target:
Human GL alpha (Galactosidase Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLα. You can calculate the concentration of Human GLα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
85-97
89-101
Average (%)
95
91
96
1:4
Range (%)
88-103
87-99
89-103
Average (%)
94
93
95
1:8
Range (%)
93-108
82-94
83-93
Average (%)
99
88
88
1:16
Range (%)
90-106
81-93
82-93
Average (%)
97
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.14
508.02
1702.58
279.56
459.52
1693.15
Standard deviation
13.58
29.47
53.46
18.31
23.99
66.03
C V (%)
5.22
5.8
3.14
6.55
5.22
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GLα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLα in samples. No significant cross-reactivity or interference between Human GLα and analogues was observed.
