Description
Human GLbeta (Galactosidase Beta) ELISA Kit
The Human GL? (Galactosidase Beta) ELISA Kit is a specialized assay designed for the quantitative detection of Galactosidase Beta levels in a variety of biological samples. Galactosidase Beta is a key enzyme involved in the breakdown of complex carbohydrates, specifically cleaving terminal galactose residues from glycoproteins. This enzyme plays a critical role in various cellular processes, including carbohydrate metabolism and glycoprotein degradation. Accurate measurement of Galactosidase Beta levels is essential for understanding its enzymatic activity and its impact on carbohydrate metabolism.
The Human GL? ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. With stringent quality control measures in place, this kit provides reliable performance and ease of use, making it an ideal choice for both research and clinical applications. Researchers can rely on Assay Genie's GL? ELISA Kit for precise and dependable quantification of this important enzyme in their studies.
| Product Name: | Human GLbeta (Galactosidase Beta) ELISA Kit |
| Product Code: | AEES00097 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 3.13-200 pg/mL |
| Sensitivity: | 1.88 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human GL? in samples. No significant cross-reactivity or interference between Human GL? and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human GL? concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GL?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GL? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GL?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GL?. You can calculate the concentration of Human GL? in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
