The Human GLRX3 (Glutaredoxin 3) CLIA Kit is a powerful tool for the precise measurement of GLRX3 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable resource for a diverse range of research studies.GLRX3, also known as glutaredoxin 3, plays a crucial role in redox regulation and is involved in various cellular processes, including cell growth, apoptosis, and the response to oxidative stress. Dysregulation of GLRX3 has been linked to a range of diseases, including cancer, metabolic disorders, and cardiovascular diseases, highlighting its significance as a potential biomarker and therapeutic target.
By accurately quantifying GLRX3 levels, researchers can gain valuable insights into disease mechanisms, identify potential therapeutic targets, and develop novel treatment strategies. The Human GLRX3 CLIA Kit offers a user-friendly and efficient solution for studying the role of GLRX3 in health and disease, making it an essential tool for cutting-edge research in the fields of molecular biology, biochemistry, and medicine.
Product Name:
Human GLRX3 (Glutaredoxin 3) CLIA Kit
SKU:
HUES00280
Target:
Human GLRX3 (Glutaredoxin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GLRX3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLRX3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLRX3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GLRX3. You can calculate the concentration of Human GLRX3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
94-108
103-116
Average (%)
96
101
109
1:4
Range (%)
92-107
86-99
89-104
Average (%)
100
92
96
1:8
Range (%)
86-99
86-100
90-103
Average (%)
92
92
96
1:16
Range (%)
96-109
84-99
90-101
Average (%)
103
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
99-116
106
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.15
315.66
752.34
104.98
337.61
726.38
Standard deviation
11.67
25.0
76.59
11.13
39.03
52.37
C V (%)
12.01
7.92
10.18
10.6
11.56
7.21
Sample type &Sample volume:
Plasma; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GLRX3 concentrations in Plasma.
Specificity:
This kit recognizes Human GLRX3 in samples. No significant cross-reactivity or interference between Human GLRX3 and analogues was observed.
