Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) CLIA Kit
The Human GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) CLIA Kit is a cutting-edge assay designed for the precise measurement of GM-CSF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.GM-CSF is a key regulator of immune responses and hematopoiesis, playing a vital role in the development and function of granulocytes and macrophages.
Dysregulation of GM-CSF has been linked to autoimmune disorders, inflammatory diseases, and certain cancers, underscoring its importance as a biomarker in disease research and drug development.With the Human GM-CSF CLIA Kit, researchers can explore the intricate role of GM-CSF in health and disease, paving the way for innovative treatments and potential therapeutic interventions. Trust Assay Genie for reliable and high-quality assay kits that drive scientific discovery forward.
Product Name:
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) CLIA Kit
SKU:
HUES00079
Target:
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GM-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GM-CSF. You can calculate the concentration of Human GM-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
94-108
96-109
Average (%)
94
102
103
1:4
Range (%)
102-116
94-108
95-112
Average (%)
110
101
103
1:8
Range (%)
89-104
98-112
95-109
Average (%)
95
105
101
1:16
Range (%)
85-97
92-108
95-113
Average (%)
90
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.15
49.91
179.96
25.64
49.29
165.28
Standard deviation
2.23
5.74
20.03
3.07
4.4
17.49
C V (%)
8.87
11.5
11.13
11.97
8.93
10.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GM-CSF in samples. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
